Understanding the effect of endocrine ageing on women’s brain chemistry, and as a risk factor for disease is of critical importance. Women are often overlooked or excluded from participant samples, which has prevented scientific understanding of how hormones impact the female brain. Oestrogen deprivation due to menopause is associated with several debilitating neurological symptoms, which can necessitate for hormone replacement treatment (HRT). My research involves the use of proton magnetic resonance spectroscopy, magnetic resonance imaging and functional magnetic resonance imaging to assess the HRT’s influence on women’s neurochemistry, brain morphology, and brain function at menopause.